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商品详细Kapa生物系统/Kapa mRNA HyperPrep试剂盒/KK8540/24文库

Kapa生物系统/Kapa mRNA HyperPrep试剂盒/KK8540/24文库

商品编号： KK8540

品牌： Kapa

市场价： ¥21600.00

美元价： 12960.00

产地：美国(厂家直采)

公司：

产品分类： RNA文库制备试剂盒

公司分类： RNA_library_preparation

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Description:

KAPARNAHyperPrepKit

KAPAmRNAHyperPrepKits

Single-DayRNA

TheKAPAmRNAHyperPrepKitsutilizenovelchemistrythatenablesthecombinationofenzymaticstepsandfewerreactionpurifications,resultinginatrulystreamlinedsolutionforthepreparationofhigh-qualitymRNA-seqlibraries.mRNAcapturebeadsareusedpriortolibrarypreparation,whichenrichesformaturemRNAovernon-polyadenylatedspecies,suchasribosomal,precursor,andnoncodingRNAs.

Thestrand-specificworkflowisflexIBLe–supportinglibraryconstructionfromlower-inputamountsanddegradedsamples.KitscontainallreagentsrequiredformRNAcaptureandlibrarypreparation,withtheexceptionofKAPAAdapters(availableseparately).Benefitsinclude:

single-daylibraryconstruction,inclusiveofmRNAcapture
reducedhands-onandoveralltimethroughfewerenzymaticandreactioncleanups
flexibleinputof50ng–1µgtotalRNA*
maintainover99%strandspecificity*
KAPAPureBeadsincludedforreactionpurifications

KAPARNAHyperPrepKits

KAPARNAHyperPrepKitsleveragethesameCDNAsynthesisandlibrarypreparationworkflowimprovementsasoutlinedfortheKAPAmRNAHyperPrepKits.KAPARNAHyperPrepKitsincludeallenzymesandbuffersrequiredforcDNAlibrarypreparation,butdonotcontainmRNAcapturereagentsorKAPAAdapters.Kitscanbeusedtopreparelibrariesfrom1ng–100ngoftotal,poly(A)-selected,orrRNA-depletedRNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable. FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapter andBeadCalculator.

DownloadourAdapterandBeadCalculator

^*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

The KAPA mRNA HyperPrep Kit results in a reduction in the total number of reads wasted due to both PCR duplicates and alignment to rRNA loci in comparison to the TruSeq and NEBNext kits. Libraries were generated in quadruplicate with 50 ng of high-quality Universal Human Reference (UHR) RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

With an equivalent amount of sequencing, more genes and unique transcripts are identified using the KAPA mRNA HyperPrep Kit in comparison to the TruSeq and NEBNext kits. Libraries were generated in quadruplicate with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

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Sequencewhatmatters

WastefewerreadsduetothecombinationofrRNAcarryoverandPCRduplicates
Identifymoreuniquetranscriptsandgeneswithequivalentsequencing

For the top 1000 transcripts, the KAPA mRNA Hyper Prep Kit results in more even coverage across transcript lengths, as assessed by both a normalized coverage plot and coverage coefficient of variation (CV), in comparison to competitor workflows. Libraries were generated with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

Improved coverage of GC-rich regions (outlined in red) of the YBX1 (rRNA depletion) and SLC2A4RG (mRNA capture) genes, along with improved splice junction and antisense transcript detection was demonstrated using the KAPA mRNA Hyper Prep Kit in comparison to competitor workflows. Libraries were generated with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

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Achievesuperiorcoverageuniformity

Obtainmoreuniformdistributionofreadsacrosstranscripts
ImprovecoverageofdifficultGC-richtranscripts

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPARNAHyperPrepKitswithRiboErase

KAPAStrandedmRNA-SeqKits

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits

Applications:

Applications

Geneexpressionanalysis
Detectionofgenefusions,isoforms,andotherstructuralvariants
Noveltranscriptidentification,includingnoncodingtranscripts
SNVdiscovery
Wholetranscriptome(KAPARNAHyperPrepKitsonly)
Targetedtranscriptome(KAPARNAHyperPrepKitsonly)

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

EnzymesandbuffersforrRNAdepletion,cDNAsynthesis,andlibrarypreparationcanbestoredforupto10monthsat-20°C.mRNAcapturereagentsandKAPAPureBeadscanbestoredforupto10monthsat4°C.(USonly)

KitsincludereagentsforRNAfragmentation,cDNAsynthesis,andlibrarypreparation.KitswithreagentsformRNAcapturearealsoavailable.

RNAHyper_Component Chart

Specifications

CompatibilityPlatform

IlluminaHiSeq,NextSeq,andMiSeq

LibraryType

RNA

StartingMaterial

RNAHyperPrepKit:High-qualitytotalRNA,mRNA,orrRNA-depletedRNA;mRNAHyperPrepKit:High-qualitytotalRNA

StartingMaterial

RNAHyperPrepKit:1ng–100ng;mRNAHyperPrepKit:25ng–1µg

品牌介绍

Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完美产品，助力生命科学研究”这一最高宗旨。产品：

公司介绍

品牌分类

DNA文库制备库放大 Illumina图书馆准备图书馆量化 RNA文库制备样品质量控制

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