4000-520-616
欢迎来到免疫在线！(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
产品库 品牌馆 现货促销 技术交流 新闻动态 联系我们
Kapa
主营：DNA扩增、二代测序、分子诊断
咨询热线电话
4000-520-616
公司首页 公司介绍 产品中心 新闻动态 联系方式 在线下单
当前位置： 首页 > 产品中心 > DNA_library_preparation > Kapa biosystems/Kapa Hyper Prep Kits/KK8500/8反应
商品详细Kapa biosystems/Kapa Hyper Prep Kits/KK8500/8反应
Kapa biosystems/Kapa Hyper Prep Kits/KK8500/8反应
Kapa biosystems/Kapa Hyper Prep Kits/KK8500/8反应
商品编号： KK8500
品牌： Kapa
市场价： ¥5440.00
美元价： 3264.00
产地： 美国(厂家直采)
公司：
产品分类： DNA文库制备试剂盒
公司分类： DNA_library_preparation
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
立即询价
商品介绍

Description:

WithKAPALibraryAmplificationPrimerMix(10X)

KAPAHyperPrepKits

Shiftyourworkflowintohyperdrive.

TheKAPAHyperPrepKitisaversatile,streamlinedsolutionforDNAlibrarypreparationforIllumina®sequencing.

Thenovelone-tubechemistry,containoptimallyformulatedandevolvedenzymesthatenablehigheryieldsofadapter-ligatedlibraryandloweramplificationbias.Thistranslatestohigherlibrarydiversity,lowerduplicationratesandmoreuniformcoverage,particularlyforFFPEandlow-inputsamples.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourAdapterandBeadCalculator

 

ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

KAPA Hyper Prep Kits convert more input DNA to adapter-ligated library. Libraries were prepared from Covaris-sheared DNA and quantified prior to library amplification with the KAPA Library Quantification Kit. The KAPA Hyper Prep Kits achieved the highest yield of adapter-ligated library across the range of inputs (1 μg, 100 ng or 10 ng), and required fewer cycles of amplification to generate 1 μg of amplified library required for target capture. Data on file.
Number of amplification cycles needed to generate ≥1 µg amplified library for target capture with the SeqCap EZ Comprehensive Cancer Design (578 genes, 4 MB; Roche Nimblegen), for libraries prepared with each kit. The higher conversion rates achieved with the KAPA Hyper Prep Kit, combined with high-efficiency, low-bias amplification with KAPA HiFi DNA Polymerase, translates to significantly less cycles of pre-capture amplification and subsequent improvements in sequence data quality. Data on file.
PrevNext

Improvelibraryyieldsandsequencequality

  • Higherlibraryyieldstranslatetogreatermolecularcomplexity
  • FewercyclesofamplificationwithKAPAHiFiDNAPolymeraseresultsinlowerduplicationratesandimprovedcoverage
Sequencing metrics FFPE libraries prepared for whole exome sequencing. Libraries were prepared from 100 ng of FFPE DNA, and exome capture was performed with the SeqCap EZ HGSC VCRome Design, Roche NimbleGen. Higher library yields enabled fewer cycles of amplification, leading to significantly lower duplication rates and higher target coverage. Data on file.
Conversion rates for FFPE DNA has been identified as a primary bottleneck in NGS library construction. With the KAPA Hyper Prep Kit, conversion rates for FFPE samples are similar to those for high-quality DNA. This leads to significantly lower duplication rates and higher overall target coverage in exome and other target capture workflows. Conversion data are for libraries prepared from 80 – 100 ng. Covaris-sheared FFPE DNA of variable quality, or 100 ng control DNA. Data on file.
PrevNext

Createhigh-qualitylibrariesfromFFPEsamples

  • Generatehigh-qualitylibrariesfrom250ngFFPEDNAorless*
  • Significantlylowerduplicationratesandhighercoverage*
The streamlined KAPA Hyper Prep workflow. End repair, A-tailing and ligation is performed in a single tube, without intervening cleanup steps. The workflow is ideally suited for robust, high-throughput sample preparation for Illumina sequencing. Data on file.

Completelibraryconstructioninlessthan3hours

  • One-tubeworkflowwithminimalcleanupstepsreducesoveralltime,andminimizeshands-ontime
  • Sample-to-libraryin<2hoursforPCR-freeworkflows,or<3hourswithamplification*
  • Fewerhandlingstepsleadtoimprovedconsistencyandreproducibility
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries were amplified with the Real-Time library Amplification Kit, prior to sequencing on an Illumina MiSeq. With the novel KAPA Hyper Prep chemistry, higher adapter concentrations can be used to improve library diversity. Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries were amplified with the Real-Time library Amplification Kit, prior to sequencing on an Illumina MiSeq. With the novel KAPA Hyper Prep chemistry, higher adapter concentrations can be used to reduce duplication rates. Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries prepared with the two kits had similar insert sizes (D). Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries prepared with the two kits had similar insert sizes (D). Data on file.
PrevNext

Improveperformancewithlow-inputsamples

  • Generatemoresequence-qualitylibrarymoleculeswithoutincreasingadapter-dimers
  • Increaseyieldswithoutinducingsizebias

 

*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

Sample QC

KAPAhgDNAQuantificationandQCKit

Library Amplification

KAPALibraryAmplificationKits

Library Quantification

KAPALibraryQuantificationKits

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

Applications:

Applications
  • WholeGenomeSequencing
  • WholeExomeSequencing
  • TargetedSequencing(custompanels)
  • ChIP-seq
  • AmpliconSequencing
  • Methyl-seq

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto12months at-20˚C.

Kitscontain KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix

Components

Hyper_Component Chart

Specifications

Spec
Description
CompatIBLePlatform
lluminaHiSeq,MiSeq,NextSeqandGAIIx
LibraryType
DNA
StartingMaterial
FragmentedDNA,CDNA,orPCRamplicons
InputAmount
1ng–1µg
品牌介绍
Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完美产品，助力生命科学研究”这一最高宗旨。产品：
公司介绍
Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完
品牌分类
DNA文库制备 库放大 Illumina图书馆准备 图书馆量化 RNA文库制备 样品质量控制
联络我们
服务热线：4000-520-616
（限工作日9:00-18:00）
QQ ：1570468124
手机：18915418616
快速导航：
总部地址：
江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125)
工作时间：周一至周五9:00~18:00
技术支持：
短信：18915418616
服务热线：
4000-520-616（全国）
☏  0512-67156496（苏州）
版权信息：
©  2015-2022 苏州蚂蚁淘生物科技有限公司 版权所有 中国
网站备案：
友情链接：
免责声明：
本站有部分内容来自互联网，如无意中侵犯了某个媒体 、公司 、企业或个人等的知识产权，请来电或致函告之，
本网站将在规定时间内给予删除等相关处理。
扫描二维码关注官方微信
获取最新促销及产品信息