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当前位置： 首页 > 产品中心 > DNA_library_preparation > Kapa生物系统/Kapa HyperPlus试剂盒/KK8514/96反应
商品详细Kapa生物系统/Kapa HyperPlus试剂盒/KK8514/96反应
Kapa生物系统/Kapa HyperPlus试剂盒/KK8514/96反应
Kapa生物系统/Kapa HyperPlus试剂盒/KK8514/96反应
商品编号： KK8514
品牌： Kapa
市场价： ¥61440.00
美元价： 36864.00
产地： 美国(厂家直采)
公司：
产品分类： DNA文库制备试剂盒
公司分类： DNA_library_preparation
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Description:

WithLibraryAmplification

KAPAHyperPlusKits

NGSlibrarypreparation. Evolved.

TheKAPAHyperPluskitprovidesastreamlinedworkflowthatincludesfragmentationandlibrarypreparationinasingletube.Buildingonindustry-leADInglibraryconstructionefficiencies,thisintegratedsolutioncombinesenzymaticfragmentation,similarinqualitytomechanicalshearing,withthespeedandconvenienceoftagmentation-basedworkflows.Benefitsinclude:

  • DNAfragmentationandlibraryprepin2.5hours*
  • FlexIBLeDNAsampleinputfrom1ng–1µg*
  • Reducedbiasandmaximizesequencecoverage*
  • PCR-freeworkflows

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

Downloadour AdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

Workflow

KAPAHyperPlusWorkflow

ProductHighlights

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Tunableandreproduciblefragmentation

  • Adjustlibraryinsertsizesfrom150–800bpbyvaryingfragmentationtime
  • ReproducibleinsertsizesacrossarangeofGCcontentandDNAinputamounts

Industryleadinglibraryyields…

  • Routinelyachieveconversionrates>80%from≥100nginput*
  • SuperiorperformanceacrossarangeofDNAinputamounts*
  • HighlibraryyieldsenablePCR-freeworkflowsfromaslittleas50ngstartingmaterial*
Single nucleotide polymorphism (SNP) detection sensitivity indicates better sequencing quality. Libraries were prepared for capture using the Nimblegen SeqCap EZ HGSC VCRome panel, 50 ng input, standard capture protocol. SNPs were called using LoFreq with default settings and a minimum per-base depth of coverage cut-off of 10. Sensitivity (true positive rate) was calculated as the ratio of true positives divided by the sum of true positives and false negatives. Total SNPs analysed was greater than 25,000. Data on file.
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…Enablesuperiorsequencingresults

  • Higherconversionratesresultsinfeweramplificationcyclesandlowerduplicationrates
  • Detectlow-frequencymutationswithhighconfidenceduetogreaterlibrarydiversityandmoreuniformsequencecoverage
GC bias comparison. GC bias for C. difficile, E. coli, B. pertussis was assessed by calculating the GC content of the reference in 100 bp bins and plotting normalized coverage across these bins for Covaris and Hyper Prep, HyperPlus, NEBNext ® Ultra ™ and Nextera ™ workflows, using Picard CollectGCBiasMetrics. Libraries were prepared from 1 ng of input DNA. In the absence of sequencing bias, all bins would be equally represented, indicated by a horizontal distribution centered on a normalized coverage of 1.0. Distribution of GC content in the genome is indicated by the grey histograms. Data on file.
GC bias comparison. GC bias for C. difficile, E. coli, B. pertussis was assessed by calculating the GC content of the reference in 100 bp bins and plotting normalized coverage across these bins for Covaris and Hyper Prep, HyperPlus, NEBNext ® Ultra ™ and Nextera ™ workflows, using Picard CollectGCBiasMetrics. Libraries were prepared from 1 ng of input DNA. In the absence of sequencing bias, all bins would be equally represented, indicated by a horizontal distribution centered on a normalized coverage of 1.0. Distribution of GC content in the genome is indicated by the grey histograms. Data on file.
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Minimalsequencecoveragebias

  • Lowersequencebiaswhencomparedtotagmentationandotherenzymaticfragmentationmethods*
  • Equivalentperformancetomechanicalshearing*
  • Lessbiasleadstomoreuniformsequencingcoverageandreducedsequencingcosts*

 

*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

Sample QC

KAPAhgDNAQuantificationandQCKit

Library Amplification

KAPALibraryAmplificationKits

Library Quantification

KAPALibraryQuantificationKits

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

Applications:

Applications
  • WholeGenomeSequencing
  • WholeExomeSequencing
  • TargetedSequencing(custompanels)
  • FFPESampleSequencing

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto6monthsat-20ºC.

KitscontainKAPAFragEnzyme,KAPAFragBuffer(10X),KAPAFragConditioningSolution,KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix.

 

 

Specifications

Spec
Description
CompatiblePlatform
lluminaHiSeq®,MiSeq®,NextSeq®
LibraryType
DNA
StartingMaterial
genomicDNA,FFPEDNA,PCRamplicons
InputAmount
1ng–1µg
品牌介绍
Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完美产品，助力生命科学研究”这一最高宗旨。产品：
公司介绍
Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完
品牌分类
DNA文库制备 库放大 Illumina图书馆准备 图书馆量化 RNA文库制备 样品质量控制
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