Kapa biosystems/Kapa-Stringed mRNA Seq试剂盒/KK8002/60毫升-免疫在线蚂蚁淘旗下平台

当前位置：首页 > 产品中心 > RNA_library_preparation > Kapa biosystems/Kapa-Stringed mRNA Seq试剂盒/KK8002/60毫升

商品详细Kapa biosystems/Kapa-Stringed mRNA Seq试剂盒/KK8002/60毫升

Kapa biosystems/Kapa-Stringed mRNA Seq试剂盒/KK8002/60毫升

商品编号： KK8002

品牌： Kapa

市场价： ¥14400.00

美元价： 8640.00

产地：美国(厂家直采)

公司：

产品分类： RNA文库制备试剂盒

公司分类： RNA_library_preparation

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Description:

KAPAPureBeads(60mL)

KAPAStrandedmRNA-SeqKits

Evendifficultmessagesshouldbeunderstood.

KAPAStrandedmRNA-SeqKitsincludealltheenzymesandbuffersrequiredforCDNAlibrarypreparationforIlluminaNext-GenerationSequencing,utilizing100ng–4µgoftotalRNA.KAPAmRNACaptureBeadsareincludedforisolationofpoly(A)-tailedRNA.Kitsprovideprecisemeasurementofstrandorientation(>99%),uniformcoverage,andhigh-confidencemappingofalternatetranscripts,andareoptimizedfortheimprovedcoverageofGC-richandlow-abundancetranscripts.*KitscontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,aswellasKAPAmRNACaptureBeadsandastreamlined,“with-bead”protocol.

KAPAStrandedRNA-SeqKitsincludealltheenzymesandbuffersrequiredforcDNAlibrarypreparationforIlluminaNext-GenerationSequencing,butdonotcontaintheKAPAmRNACaptureBeads.Kitscanbeusedtopreparelibrariesfrom10–400ngofeitherpoly(A)-selected,ribosomallydepleted,ortotalRNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

Download AdapterandBeadCalculator

_*Dataonfile.ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

Improved coverage of GC-rich transcripts. The 5

Uncoverchallengingtranscripts

ImprovedcoverageofGC-richtranscripts
Enhancedidentificationofexonicregions

Improved coverage of low-abundance transcripts. GLTPD1, a lesser-expressed transcript, is covered more comprehensively with the KAPA Stranded mRNA-Seq Kit (green) at 500 ng input total RNA (outlined in red). In comparison, Illumina TruSeq Stranded mRNA Sample Prep Kit (orange) shows coverage gaps, even with higher inputs (4 μg). Data on file.

Detectlow-abundancetranscripts

Enablesidentificationoftranscriptsmissedbycompetitorkits,evenwithhighinput*
Highuniformityacrossvaryingamountsofsampleinput*

30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile."> Highly mapped reads and strand specificity enable sensitive detection of expressed genes. With similar numbers of filter-passed reads (>30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile.

Highly mapped reads and strand specificity enable sensitive detection of expressed genes. With similar numbers of filter-passed reads (>30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile.

Identifymoregenes

HigherpercentageofuniquelymappedreadscomparedtoIllumina TruSeq™StrandedmRNASamplePrepKits*
Lowerduplicationratesyieldbettercoverage

Minimal positional bias. With intact, high-quality RNA input, 3

Maintainhighcoverageuniformity

Minimal5’–3′biasacrosstranscripts
Moreuniformdistributionofreadsovereachtranscript

_*Dataonfile.

RelatedProducts

SequencingchallengingRNAsamples?HowaboutDNA?CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAhgDNAQuantificationandQCKit

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits

Applications:

Applications

Geneexpression
Singlenucleotidevariation(SNV)discovery
Post-transcriptionalSNVs
Fusiongeneidentification
Targetedtranscriptome
Wholetranscriptome

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto8 months at-20˚C. KAPAmRNACaptureBeadscanbestoredforupto8months at-4˚C.

Components

mRNA_Component Chart

Specifications

CompatIBLePlatform

IlluminaHiSeq,NextSeq,MiSeqandGAIIx

StartingMaterial

TotalRNA,mRNA,orribosomaldepletedRNA

InputAmount

StrandedmRNA-SeqKits:100ng–4µgStrandedRNA-SeqKits:10ng–400ng

品牌介绍

Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完美产品，助力生命科学研究”这一最高宗旨。产品：

公司介绍

品牌分类

DNA文库制备库放大 Illumina图书馆准备图书馆量化 RNA文库制备样品质量控制

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：https://sequencing.roche.com/en-us.html