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当前位置： 首页 > 产品中心 > RNA_library_preparation > Kapa生物系统/Kapa链RNA序列，带核糖酶/KK8722/96适配器，每个适配器x 20µl
商品详细Kapa生物系统/Kapa链RNA序列，带核糖酶/KK8722/96适配器，每个适配器x 20µl
Kapa生物系统/Kapa链RNA序列，带核糖酶/KK8722/96适配器，每个适配器x 20µl
Kapa生物系统/Kapa链RNA序列，带核糖酶/KK8722/96适配器，每个适配器x 20µl
商品编号： KK8722
品牌： Kapa
市场价： ¥46080.00
美元价： 27648.00
产地： 美国(厂家直采)
公司：
产品分类： RNA文库制备试剂盒
公司分类： RNA_library_preparation
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Description:

KAPADual-IndexedAdapterKit,(15µM)

KAPAStrandedRNA-SeqwithRiboErase

Evolvedtofocus.

TheKAPAStrandedRNA-SeqKitwithRiboEraseoffersahigh-quality,comprehensivesolutionfortranscriptomesequencing.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablessuperiorreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).KitsalsocontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,andincludeastreamlined,“with-bead”protocol.

  • Upto99.98%rRNAdepletion*
  • FlexIBLeinputof100ng–1μgtotalRNAfromhuman,mouse,orratspecies*
  • Robustandreproducibleacrossvariousinputamounts
  • Automation-friendlyworkflow

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourAdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

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IndustryleADIngrRNAdepletion

  • SuperiorrRNAdepletionfromhigh-qualityandFFPEsamples*
  • MoreeconomicalNGSsequencingduetodecreasedrRNAreads,providingdeepersequencingoftranscriptsofinterest
High-quality sequencing data obtained from KAPA Stranded RNA-Seq Kit with RiboErase when compared to the equivalent Illumina or NEB kits. With equivalent mapping rates, KAPA Stranded RNA-Seq Kit with RiboErase provides lower duplication rates, better coverage uniformity and consistently detects more unique transcripts and genes. This enables a more sensitive detection of the expressed genes within the sample. Data on file.
Improved coverage of GC-rich transcripts. IGV image showing coverage and splice junction tracks of the haemoglobin alpha transcript (HBA1) which has a GC content of 62.8%. The GC content track shows GC-rich regions in red, and AT-rich regions in blue. More even coverage, improved splice-site recognition, and more representative plot compared to the undepleted sample (red) is seen when libraries are prepared with the KAPA Stranded RNA-Seq Kit with RiboErase (green) compared to the equivalent Illumina (orange) or NEB (blue) kits. Data on file.
Better detection of low-abundance transcripts. IGV image showing coverage and splice junction tracks of MALAT1, a low abundance long non-coding RNA transcript, which is more comprehensively covered with use of KAPA Stranded RNA-Seq Kit with RiboErase (green) for library preparation. In comparison, Illumina Ribo-Zero Gold (orange) and NEBNext (blue) show less even coverage of this transcript. Data on file.
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Unsurpassedsequencingdataquality

  • Detectionofmoregenesanduniquetranscripts
  • Accurateandclearidentificationofsplicesitesandalternativegenesplicing
  • ImprovedcoverageenablingbetterdetectionofdifficultandGC-richtranscripts
Hierarchically clustered heatmap showing the correlations of gene expression levels between different laboratories and RNA input amounts. All correlations were greater than 0.93, with technical replicates produced in the same lab exceeding 0.98. Generally, all libraries from a single lab, including those produced from different input amounts, clustered together. The data indicates that the KAPA Stranded RNA-Seq Kit with RiboErase delivers robust, reproducible, and unbiased gene expression data from a wide range of input amounts. Data on file.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
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Highlyreproduciblesequencingresults

  • Highcorrelationevenbetweendifferenttestingconditions
  • Lowsample-to-samplevariationformorereliableresults
Improved coverage of the whole transcript. The housekeeping gene Beta-Actin has improved coverage at the 3’ and 5’ regions using the KAPA Stranded RNA-Seq Kit with RiboErase (green) when compared to the equivalent Illumina Ribo-Zero Gold (orange) and NEBNext (blue) kits. Data on file.
Minimal positional bias. With 100 ng of high-quality RNA input, 3
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Improvedcoverageuniformity

  • Uniformdistributionofreadsovereachtranscript
  • Minimal5’–3’biasacrosstranscripts


*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

KAPA RNA HyperPrep Kits with RiboErase

KAPARNAHyperPrepKitswithRiboErase

KAPA mRNA HyperPrep Kits

KAPAmRNAHyperPrepKits

Library Amplification

KAPALibraryAmplificationKits

Library Preparation

KAPAHyperPrepKits

Library Quantification

KAPALibraryQuantificationKits

Applications:

Applications
  • WholeTranscriptome
  • GeneExpression
  • TranscriptDiscoveryandAnnotation
  • TotalRNA-Seq

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto10monthsat-20ºC.

KitscontainKAPAHybridizationBuffer,KAPAHybridizationOligos(Human/Mouse/Rat),KAPADepletionBuffer,KAPARNaseH,KAPADNaseBuffer,KAPADnase,KAPAFragment,PrimeandEluteBuffer(2X),KAPA1stStrandSynthesisBuffer,KAPAScript,KAPA2ndStrandMarkingBuffer,KAPA2ndStrandSynthesisEnzymeMix,KAPAA-TailingBuffer(10X),KAPAA-TailingEnzyme,KAPALigationBuffer(5X),KAPADNALigase,KAPAPEG/NaClSPRISolution,KAPALibraryAmplificationPrimerMix(10X)andKAPAHiFiHotStartReadyMix(2X).

Components

RIBO-Component Chart

Specifications

Spec
Description
CompatiblePlatform
IlluminaHiSeq®,NextSeq®andMiSeq®
LibraryType
RNA
StartingMaterial
High-qualityTotalRNA
InputAmount
100ng-1µg
品牌介绍
Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完美产品，助力生命科学研究”这一最高宗旨。产品：
公司介绍
Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完
品牌分类
DNA文库制备 库放大 Illumina图书馆准备 图书馆量化 RNA文库制备 样品质量控制
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