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当前位置： 首页 > 产品中心 > RNA_library_preparation > 含核糖酶（HMR）/KK8721/25 mL的Kapa生物系统/Kapa RNA HyperPrep试剂盒
商品详细含核糖酶（HMR）/KK8721/25 mL的Kapa生物系统/Kapa RNA HyperPrep试剂盒
含核糖酶（HMR）/KK8721/25 mL的Kapa生物系统/Kapa RNA HyperPrep试剂盒
含核糖酶（HMR）/KK8721/25 mL的Kapa生物系统/Kapa RNA HyperPrep试剂盒
商品编号： KK8721
品牌： Kapa
市场价： ¥3460.00
美元价： 2076.00
产地： 美国(厂家直采)
公司：
产品分类： RNA文库制备试剂盒
公司分类： RNA_library_preparation
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Description:

KAPAAdapterDilutionBuffer(25mL)

KAPARNAHyperPrepKitswithRiboErase(HMR)

Single-DayRNA

TheKAPARNAHyperPrepKitswithRiboErase(HMR)utilizenovelchemistrythatenablesthecombinationofenzymaticstepsandfewerreactionpurifications,resultinginatrulystreamlinedsolutionforthepreparationofhigh-qualityrRNA-depletedRNA-seqlibraries.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablesefficientreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).Thestrand-specificworkflowisflexIBLe–supportinglibraryconstructionfromlower-inputamountsanddegradedsamples.KitscontainallreagentsrequiredforrRNAdepletionandlibrarypreparation,withtheexceptionofKAPAAdapters(availableseparately).Benefitsinclude:

  • single-daylibraryconstruction,inclusiveofrRNAdepletion
  • reducedhands-onandoveralltimethroughfewerenzymaticandreactioncleanups
  • flexibleinputof25ng–1µgtotalRNAfromhuman,mouse,orratspecies*
  • highersuccessrateswithlowerinputanddegradedsamples
  • maintainover99%strandspecificity*
  • KAPAPureBeadsincludedforreactionpurifications

NEW!KAPADual-IndexedAdapterKitsarenowavailable. FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.DownloadourAdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

The KAPA RNA Hyper Prep Kit with RiboErase (HMR) results in a reduction in the total number of reads wasted due to both PCR duplicates and alignment to rRNA loci in comparison to the TruSeq and NEBNext kits. Libraries were generated in quadruplicate with 25 ng of high-quality Universal Human Reference (UHR) RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
Better utilize sequencing capacity. (B) Libraries were generated in quadruplicate with 25 ng (rRNA depletion) and 50 ng (mRNA capture) of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Data on file.
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Sequencewhatmatters

  • WastefewerreadsduetothecombinationofrRNAcarryoverandPCRduplicates
  • Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
Improved coverage uniformity. For the top 1000 transcripts, the KAPA RNA HyperPrep workflows result in more even coverage across transcript lengths, as assessed by both a normalized coverage plot and coverage coefficient of variation (CV), in comparison to competitor workflows. (A) Libraries were generated with 25 ng (rRNA depletion) and 50 ng (mRNA capture) of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Data on file.
Improved coverage uniformity. For the top 1000 transcripts, the KAPA RNA HyperPrep workflows result in more even coverage across transcript lengths, as assessed by both a normalized coverage plot and coverage coefficient of variation (CV), in comparison to competitor workflows. (B) Improved coverage of GC-rich regions (outlined in red) of the YBX1 (rRNA depletion) and SLC2A4RG (mRNA capture) genes was demonstrated using the Kapa workflows. Data on file.
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Achievesuperiorcoverageuniformity

  • Obtainmoreuniformdistributionofreadsacrosstranscripts
  • ImprovecoverageofdifficultGC-richtranscripts
Identify more genes and transcripts. With equivalent sequencing, the KAPA RNA HyperPrep Kit with RiboErase (HMR) in comparison to competitor workflows identified more genes and transcripts. Data on file.
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Generatehigh-qualitylibrariesfromdegradedsamples

  • Inputaslittleas25ngwithFFPEsamples,dependingontotalRNAquality
  • Achievelowduplicationratesandhighlyefficient,reproduciblerRNAremovalwithdegradedsamples
  • Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
High-level of agreement between paired FFPE and fresh frozen expression data, in transcripts per million (TPM). Pearson correlation coefficients show a higher degree of agreement with the KAPA RNA Hyper Prep Kit with RiboErase in comparison to the TruSeq Stranded Total RNA Library Prep with Ribo-zero Gold and NEBNext Ultra Directional Library Preparation with the rRNA Depletion Kit. Libraries were generated in duplicate using 100 ng inputs of paired FFPE-derived and fresh frozen breast tumor total RNA samples using the manufacturers’ standard recommendations per workflow. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
High-level of agreement between paired FFPE and fresh frozen expression data, in transcripts per million (TPM). Pearson correlation coefficients show a higher degree of agreement with the KAPA RNA Hyper Prep Kit with RiboErase in comparison to the TruSeq Stranded Total RNA Library Prep with Ribo-zero Gold and NEBNext Ultra Directional Library Preparation with the rRNA Depletion Kit. Libraries were generated in duplicate using 100 ng inputs of paired FFPE-derived and fresh frozen breast tumor total RNA samples using the manufacturers’ standard recommendations per workflow. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
High-level of agreement between paired FFPE and fresh frozen expression data, in transcripts per million (TPM). Pearson correlation coefficients show a higher degree of agreement with the KAPA RNA Hyper Prep Kit with RiboErase in comparison to the TruSeq Stranded Total RNA Library Prep with Ribo-zero Gold and NEBNext Ultra Directional Library Preparation with the rRNA Depletion Kit. Libraries were generated in duplicate using 100 ng inputs of paired FFPE-derived and fresh frozen breast tumor total RNA samples using the manufacturers’ standard recommendations per workflow. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.
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Achievereliableresultswithdegradedinputs

  • Attainahigh-degreeofexpressioncorrelationbetweenpairedFFPEandfreshfrozensampleswhichprovidesincreasedconfidenceinsequencedataaccuracy

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPA mRNA HyperPrep Kits

KAPAmRNAHyperPrepKits

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

Library Amplification

KAPALibraryAmplificationKits

Library Preparation

KAPAHyperPrepKits

Library Quantification

KAPALibraryQuantificationKits

Applications:

Applications
  • Wholetranscriptome
  • Geneexpressionanalysis
  • Detectionofgenefusions,isoforms,andotherstructuralvariants
  • Noveltranscriptidentification,includingnoncodingtranscripts
  • SNVdiscovery

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

EnzymesandbuffersforrRNAdepletion,CDNAsynthesis,andlibrarypreparationcanbestoredforupto10monthsat-20°C.KAPAPureBeadscanbestoredforupto10monthsat4°C.(USonly)

KitscontainHybridizationBuffer,HybridizationOligos(HMR),DepletionBuffer,RNaseH,DNaseBuffer,DNase,Fragment,PrimeandEluteBuffer(2X),1stStrandSynthesisBuffer,KAPAScript,2ndMarkingBuffer,2ndStrandSynthesis&A-TailingEnzymeMix,LigationBuffer,DNALigase,PEG/NaClSolution,KAPAPureBeads,LibraryAmplificationPrimerMix(10X),andKAPAHiFiHotStartReadyMix(2X).

RNAHyperRibo_Component Chart

Specifications

Spec
Description
CompatiblePlatform
0616
LibraryType
RNA
StartingMaterial
High-qualityanddegradedtotalRNA
InputAmount
25ng–1µg
品牌介绍
Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完美产品，助力生命科学研究”这一最高宗旨。产品：
公司介绍
Kapa biosystems成立于2006年，其生产基地位于南非开普敦。致力于研发、生产下一代PCR（Next-Generation PCR）所需的生物酶。分子进化技术（酶工程）打破了现有的研究局限，为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合，执着于“科技创造完
品牌分类
DNA文库制备 库放大 Illumina图书馆准备 图书馆量化 RNA文库制备 样品质量控制
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