KAPA HyperPlus/SeqCap EZ workflow: Improving Data Quality and...

来自 : 发布时间：2025-06-14

KAPA HyperPlus/SeqCap EZ workflow: Improving Data Quality and Turnaround Times for Targeted Next-Generation Sequencing of FFPE DNA - 百度学术 摘要： Figure 1. Comparative sequencing metrics. A) Duplication rates were lower for the majority of libraries prepared with the HyperPlus workflow. The average % duplicates for FFPE libraries (60.0%) represented a 26.3% decrease compared to the original protocol. As expected, the improvement in average duplication rate (23.0% vs. 27.8%) was smaller for high-quality blood samples, which have a lower intrinsic duplication rate. B) Consistent on-target rates were achieved for all libraries, and across both sample types. With the HyperPlus workflow, the average % unique on-target reads was 65.3% and 64.7% for FFPE and paired normal libraries, respectively. This equates to a 5.9% and 4.1% improvement over the original protocol. 展开 我们已与文献出版商建立了直接购买合作。 你可以通过身份认证进行实名认证，认证成功后本次下载的费用将由您所在的图书馆支付 您可以直接购买此文献，1~5分钟即可下载全文，部分资源由于网络原因可能需要更长时间，请您耐心等待哦~